Immagine
 Trilingual World Observatory: italiano, english, română. GLOBAL NEWS & more... di Redazione
   
 
\\ Home Page : Articolo
Tethering and stretching DNA.
By Admin (from 10/04/2011 @ 14:00:29, in en - Science and Society, read 1680 times)

Scientists recently discovered that DNA can be used as a molecular scaffold to make metal contacts to organic semiconductors. A key step in this process involves being able to tether the DNA to various surfaces and stretch the molecule to varying lengths.

Zhenan Bao and colleagues' new strategy involves synthesizing hybrid DNA-organic molecule-DNA (DOD) structures, then stretching and tethering the DOD assemblies between two microscopic metal electrodes. The researchers then make metal electrode-organic molecule-metal electrode (MOM) structures by further metallizing the DNA segments within the DOD structures.

The team then exploited so-called biotin-Streptavidin linkage chemistry to tether the DNA assemblies to device surfaces (quartz in this case). The basic steps are as follows: functionalizing the surface with amine (-NH2) terminated silanes; reacting the amines with N-hydroxysuccinimide (NHS) functionaliszed polyethylene glycol (PEG) chains terminated with biotin; and using Streptavidin to create a link to biotin-terminated DNA molecules.

A crucial step

"We have made progress in synthesizing these DOD hybrid structures and have now developed a reproducible surface chemistry technique to tether DNA molecules of different lengths to substrate surfaces," team member Guihua Yu told nanotechweb.org. "We have also developed a shear flow processing method to control DNA stretching and alignment. This represents a crucial step in making large-scale nanoelectronic devices based on DOD array structures."

Aside these practical applications, the technique could also be used to study single DNA molecules and how they rotate. DNA tethering and stretching may also help in manipulating nucleotides in single DNA molecules for genetic applications and to study how DNA reacts with proteins at the single-molecule level, said Yu. Other single-molecule technologies, such as DNA sequencing could also benefit.

Spurred on by these first results, the team is now working on a controllable, double-tethering process while developing in situDNA metallization for ultimately making larger-scale nanoelectronic devices based on single organic molecules. "We will then perform electrical transport measurements on these device arrays to probe how charge travels through single molecules with different chemical functionalities and length," added Yu.

The work was reported in ACS Nano.
Source: Nanotechweb.org

Articolo Articolo  Storico Storico Stampa Stampa  Share
Cannabis seeds, Autoflowering seeds, Greenhouse, Sweet Seeds, Dutch Passion
comments powered by Disqus
 
Nessun commento trovato. No comments found. Nici un comentariu găsit.

Anti-Spam: dial the numbers CAPTCHA
Text (max 5000 characters)
Nome - Name - Nume
Link ( OPTIONAL - visible on the site - NOT a must )


Disclaimer
Tuo commento sarŕ visibile dopo la moderazione. - Your comment will be visible after the moderation. - Comentariul tău va fi vizibil după moderare.
Ci sono 1899 persone collegate

< dicembre 2020 >
L
M
M
G
V
S
D
 
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
     
             

Titolo
en - Global Observatory (605)
en - Science and Society (594)
en - Video Alert (346)
it - Osservatorio Globale (503)
it - Scienze e Societa (555)
it - Video Alerta (132)
ro - Observator Global (399)
ro - Stiinta si Societate (467)
ro - TV Network (149)
z - Games Giochi Jocuri (68)

Catalogati per mese - Filed by month - Arhivate pe luni:

Gli interventi piů cliccati

Ultimi commenti - Last comments - Ultimele comentarii:
Hi, it's Nathan!Pretty much everyone is using voice search with their Siri/Google/Alexa to ask for services and products now, and next year, it'll be EVERYONE of your customers. Imagine what you are ...
15/01/2019 @ 17:58:25
By Nathan
Now Colorado is one love, I'm already packing suitcases;)
14/01/2018 @ 16:07:36
By Napasechnik
Nice read, I just passed this onto a friend who was doing some research on that. And he just bought me lunch since I found it for him smile So let me rephrase that Thank you for lunch! Whenever you ha...
21/11/2016 @ 09:41:39
By Anonimo


Titolo





05/12/2020 @ 16:30:45
script eseguito in 789 ms