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Articoli del 14/05/2012

Rezultatele studiilor lor sugereaza faptul ca problemele legate de controlul cantitatii de zahar din sânge încep la nivelul intestinelor.

Oamenii de ştiinţă au descoperit adevărata origine a diabetului

Pe baza experimentelor realizate pe soareci de laborator, cercetatorii americani considera ca se poate rescrie istoria diagnosticarii diabetului, una dintre cele mai temute si frecvente maladii moderne. Astfel, se pare ca originea diabatului este în sistemul gastro-intestinal si nu în organe precum pancreasul si ficatul, dupa cum se credea pâna acum în mediul medical.

În cadrul noului studiu, oamenii de stiinta au studiat exemplarele de soareci ale caror organisme nu erau capabile sa secrete o enzima numita acid gras-sintaza (FAS) la nivelul intestinelor. FAS este enzima esentiala pentru producerea de lipide, fiind reglata de insulina. Soarecii care nu prezentau aceasta enzima în intestine s-au îmbolnavit, în prima instanta, de inflamatie cronica intestinala, o afectiune care prezice aproape întotdeauna instalarea diabetului.

"FAS ajuta la mentinerea mucoasei intestinale într-o stare intacta, respectiv functionala. Fara aceasta enzima, bacteriile periculoase invadeaza celulele din intestinul subtire si din colon, producând inflamarea acestora, fapt care contribuie la rezistenta la insulina si instalarea diabetului", declara profesorul Xiaochao Wei, cercetator în cadrul Sectiei de Endocrinologie din cadrul universitatii americane.

Inflamarea intestinelor si rezistenta la insulina sunt afectiuni care se sustin una pe cealalta. Substantele asociate cu procesul inflamator duc la rezistenta la insulina si inhiba producerea acesteia, iar ambele fenomene interfereaza cu reglarea nivelului de zahar din sânge.

Labrintul intestinal este un mediu extrem de important pentru aparitia si dezvoltarea diabetului, deoarece conform cercetarilor, marea majoritate a suferinzilor de diabet nu prezinta doar un nivel scazut al insulinei, ci au frecvent disfunctii gastro-intestinale.

Sursa: Medical Express - via Descopera.ro

 

The protein is an enzyme that Plasmodium falciparum, the protozoan that causes the most lethal form of malaria, uses to make cell membrane.

The protozoan cannot survive without this enzyme, but even though the enzyme has many lookalikes in other organisms, people do not make it. Together these characteristics make the enzyme an ideal target for new antimalarial drugs.

The research was published in the January 6 issue of the Journal of Biological Chemistry (JBC) as "Paper of the Week" for that issue.

A cartoon based on the electron density map makes it easier to see the protein’s structure and figure out how it works. The enzyme’s job is to add a methyl group — three times — to a starting molecule as part of a process for making cell membranes. In this cartoon, the phosphate is a stand-in for the starting molecule and the green molecule is the one that donates a methyl group. Both are positioned in the active site of the enzyme, the pocket where the chemistry takes place.

The work also will be featured in ASBMB Today (the newsletter of the American Society for Biological Molecular Biology, which publishes JBC), and it will be the topic of a JBC podcast.

Sweating the cold room

The protein's structure might have remained an enigma, had it not been the "unreasonable optimism" of Joseph Jez, PhD, associate professor of biology in Arts & Sciences, which carried his team through a six-year-long obstacle course of failures and setbacks.

"What my lab does is crystallize proteins so that we can see what they look like in three dimensions," Jez says. "The idea is that if we know a protein's structure, it will be easier to design chemicals that would target the protein's active site and shut it down," Jez says.

The lastest discovery is the culmination of a project that began years before when Jez was working at the Danforth Plant Science Center in St. Louis and collaborating with scientists at the local biotech startup Divergence.

"At the time, C. elegans had just been sequenced and the Divergence scientists were looking at using it as an easy model to work out the biochemistry of parasitic nematodes," Jez says.

C. elegans is a free-living nematode, or microscopic roundworm, but many nematodes are parasitic and cause disease in plants, livestock and people.

During this project, Lavanya Palavalli, a summer intern working with Jez, crystallized the C. elegans version of the enzyme. The job of the enzyme, phosphoethanolamine methyltransferase, thankfully abbreviated to PMT, is to add methyl groups to a starting molecule, phosophoethanolamine.

"When Soon Goo Lee later took up the project," says Jez, "the plan was to try to grow better crystals of the C. elegans protein, ones good enough to get readable X-ray diffraction patterns.

Two years later, the crystals were looking better but still not good enough.

So Jez suggested that Lee go after homologous (look-alike) proteins in other organisms. "Even though the proteins are homologous, each has a different amino acid sequence and so will behave differently in the crystallizations," Jez says. "Lee went from working with two C. elegans proteins to three plant proteins, two other nematode proteins and then the Plasmodium protein," Jez says.

"He took all six of those PMT versions into the crystallization trials to maximize his odds," Jez says.

"To crystallize a protein," Jez says, "we put a solution of a salt or something else that might work as a desiccant in the bottom of a small well. And then we put a drop of our liquid protein on a microscope cover slip and flip it over the top of the well, so the drop of protein is hanging upside down in the well."

"What we're trying to do is to slowly withdraw water from the protein. It's exactly like making rock candy, only in that case, the string hanging into the jar of sugar solution helps to withdraw water," he says.

The difference is that sugar wants to form crystals and proteins are reluctant to do so.

"There are 24 wells to a tray, and we usually screen 500 wells per protein at first," Jez says. "Lee had eight proteins and so his first pass was to screen 4,000 conditions. And then he had to try different combinations of ligands to the proteins and crystallize those. This is why it took a few years to finally get where he needed to go."

Road trip!

The scientists need crystals — preferably nice, big ones —to stick in the path of an X-ray beam at Argonne National Laboratory in Chicago. (If the crystal is a good one, and all the atoms are lined up in a repeating array, the scattered X-rays will produce a clear pattern of spots.)


Soon Goo Lee, a doctoral candidate at Washington University in St. Louis, about to click the mouse button to see whether six years of work will pay off and he will be the first to see the structure of a protein no one has ever seen before.

Embedded in that pattern is the mathematical information needed to back-calculate to the position of the atoms in the protein, a process a bit like throwing a handful of pebbles in a lake and then calculating where they landed by the pattern of waves arriving at the shoreline.

Lee got the PMT from Haemonchus contortus to crystallize first, but there were technical issues with the diffraction pattern that would have made solving it technically and computationally very demanding.

"When the Plasmodium enzyme finally crystallized, Soon got four crystals kind of stacked on top of each other and each of them was paper thin," Jez says.

"I never thought it would work, but we took them to Argonne anyway and he actually did surgery under the microscope and cracked off a little tiny piece of it."

To everyone's surprise, he got a clean diffraction pattern from the crystal. "Because the Plasmodium enzyme was the smallest one and the easiest to work on, we pushed that one first," Jez says.

The moment of truth

"Once we had a Plasmodium crystal that was diffracting really well, we could try back-calculating to see whether we could extract the atom positions from the data," Jez says.

After the computer finished its calculations, Lee clicked a mouse button to see the results, which would reveal whether his years of work finally would pay off.

When Lee clicked the mouse, he got an electron density map in exceptionally sharp focus.

"When you see a map like that, it's like suddenly the wind has kicked up and you're sailing free," Jez says, "because there's this moment, like, before you click that button, no one has ever seen how this protein is put together in three dimensions. You're the first person to ever see it.

"The irony of it is we got such good quality diffraction pattern and electron density maps off such an ugly crystal," he says.

Lock and load

"Once you have the electron density map, the task is to build a structure that matches the amino acid sequence of the protein," Jez says.

"The first thing you do is put in the amino acid backbones and connect them together to form a chain. It's like having a long thread, each inch of which is an amino acid, and your job is to take that thread and move it in three dimensions through that electron density map."

The next step is to add the side chains that make one amino acid different from another, Jez says. "The amino acid sequence is known," he says. "Your goal is to match the way you string together the amino acids in the electron density map to that sequence."

"Once you have the overall structure, you can start to figure out how the enzyme works. The PMT enzyme is trying to join two molecules," Jez says. "To do that, it has to lock them in place so that the chemistry can happen, and then it has to let go of them.

"We think the protein has a lid that opens and closes," he says. "The active site stays open until the substrates enter, and then the lid clamps down, and when it clamps down it actually puts the substrates together."

Not only do infections by Plasmodium falciparum cause the most severe form of malaria, about 40 percent of the human population lives in areas where the parasite is endemic. Moreover, drugs that used to be effective against malaria are beginning to fail, in part because widespread drug counterfeiting has led to resistance.

New anti-malarial drugs are desperately needed, and the PMT protein is an ideal target. If PMT is disabled, the protozoan can't make cell membranes and it dies. Moreover, a drug that would kill Plasmodium might have minimal side effects on patients.

Although the process of identifying compounds that would target PMT is in the early stages, a handful of anti-parasitical compounds used to treat diseases are known to block PMT as well.

As for Lee, he has had a hard go of it, but now things are breaking his way. Plasmodium PMT is giving up its secrets, and the plant and nematode PMTs are coming along as well.

When he clicked the mouse button and a clean electron density map came up, he says, it was like seeing "the light at the end of a five-year-long tunnel."

Source: Washington University in St. Louis

 

Sembrerebbe un destino segnato, quello di Michelangelo. Le testimonianze sulla vita dell' artista, infatti, risalgono addirittura al suo primo giorno di vita: il neonato aveva appena emesso il suo primo vagito, che il padre Ludovico Buonarroti si era già affrettato a scrivere di suo pugno una nota in cui ricordava la nascita del suo secondogenito. Come ebbe a precisare con scrupolo, nel comune di Caprese in cui Ludovico era podestà, il calendario fiorentino segnava lunedì 6 marzo dell'anno 1474 (1475 secondo la notazione corrente).

Tuttavia, nonostante il prestigioso incarico paterno, la famiglia Buonarroti era travagliata da difficoltà economiche. Fu per questo che ben presto si trasferirono a Settignano, un paesino nei pressi di Firenze dove il piccolo Michelangelo entrò per la prima volta in contatto con la scultura. E non in senso figurato, visto che lì vivevano intere generazioni di scultori e scalpellini. Tanto che più tardi l'artista si fece vanto di essere stato allevato là dove il latte era impastato con la polvere di marmo.

E fu solo grazie alla tenacia del giovane Michelangelo che il padre rinunciò a imporgli la carriera militare o ecclesiastica. All'età di 12 anni entrò nella bottega di Domenico Ghirlandaio, dove Ludovico lo condusse di persona, non tanto per soddisfare i sogni del figlio quanto per arrotondare il bilancio familiare con il compenso da apprendista. Comunque fosse, il giovane Buonarroti si rivelò un talento tanto precoce da far esclamare al maestro: “ Costui ne sa più di me”.

Il Ghirlandaio non sbagliava. Dopo tre anni di apprendistato, Michelangelo ne sapeva abbastanza da trasferirsi a Firenze presso il giardino di San Marco,dove Lorenzo il Magnifico ospitava e sovvenzionava gli artisti più promettenti. Fu proprio qui che il giovane scultore impressionò il mecenate con la sua permalosità: il principe gli aveva fatto notare la dentatura troppo perfetta sulla statua di un vecchio fauno. Buonarroti colse la provocazione e in un batter d'occhio fece saltare un dente e con lo scalpello ne bucò un altro.

Ad attirare l'attenzione del cardinale e collezionista Raffaele Riario fu invece una copia di un antico Cupido eseguita dallo stesso Michelangelo, e che il prelato aveva acquistato a peso d'oro – all'insaputa dell'artista – come reperto archeologico. Dopo aver scoperto l'inganno e sbollito la rabbia, il cardinale volle a tutti i costi conoscere l'artefice di quell'opera meravigliosa. E ne fece la fortuna.

Così, nel 1496 l’artista si trasferì a Roma dove produsse alcune delle sue opere più grandi. Dopo la Pietà, scolpita a soli 22 anni, Michelangelo si dedicò anche agli affreschi della Cappella Sisitina (1508-1512) e al Giudizio Universale (1534-1541). Ma nei suoi lunghi anni di attività l'artista continuò anche a scolpire altri capolavori tra cui il David (1501) e il Mosè (1513-1515).

Per raccontare tutta la vita dell'artista non basterebbe un libro. Fatto sta che Michelangelo divenne uno degli artisti più famosi dell'epoca. Gli storici raccontano che morì a Roma il 18 febbraio 1564, all'età di 88 anni: fino a pochi giorni prima aveva lavorato di scalpello sull'incompiuta Pietà Rondanini.

Fonte: wired.it

 

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There's lots of anecdotal evidence of THC/cbd actually curing cancer. The beauty of cb1 and cb2 receptors.
03/04/2014 @ 13:35:53
By Craig Daniel Smith
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03/04/2014 @ 12:29:32
By Brandon Smith
I concur, Ginamarie. I was going to say pretty much the same thing.
03/04/2014 @ 12:27:16
By Higino Alexandre
our best focus - in my opinion isn't focusing on paying for the funding for research - but freeing the system up to do the research - we need to get it out of schedule I - get it off the hit list so t...
03/04/2014 @ 12:26:30
By Ginamarie Pezzi
understanding the miraculous mechanics of how cannabinols work truly at the heart of nature's beautiful way
02/04/2014 @ 23:26:12
By Doc O'Zee
Scientists studying the benefits of cannabis are just finding out now what most pot smokers have known about for thousands of years. Of course, this is due to the prohibition of cannabis of the last 7...
02/04/2014 @ 20:48:14
By Higino Alexandre
Marijuana is a miracle plant.
02/04/2014 @ 18:46:17
By Mark Garnett


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